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Product Name
CHRAC1 Polyclonal Antibody
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Description
Polyclonal antibody to CHRAC1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHRAC1 antibody; CHARC1 antibody; CHARC15 antibody; CHRAC-1 antibody; CHRAC-15 antibody; CHRAC15 antibody; YCL1 antibody; chromatin accessibility complex 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human CHRAC1 (NP_059140.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CHRAC1 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and CHRAC1 knockout HeLa cells, using CHRAC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"