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Product Name
Choline kinase alpha-Specific antibody
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Description
Choline kinase alpha-Specific Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, COLO 320 cells, human testis tissue, mouse colon tissue, mouse testis tissue, Raji cells. Positive IHC detected in human breast cancer tissue, human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHETK alpha antibody; CHK antibody; CHKA antibody; CHKA-Specific antibody; Choline kinase alpha antibody; CKI antibody; Ethanolamine kinase; antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001277). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109241(CHKA-Specific antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using CHKA antibody Catalog No:109241 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:109241(CHKA-Specific Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:109241(CHKA-Specific Antibody) at dilution of 1:50(under 40x lens)
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Background
Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.
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