CHMP1A Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human brain tissue, human lung tissue, human placenta tissue, human skin tissue. Positive IF detected in HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, mouse kidney tissue, mouse lung tissue. Observed molecular weight by Western-blot: 33 kDa ,25 kDa
ELISA, WB, IHC, IP, IF
Human, Mouse; other species not tested.
CHMP1 antibody; CHMP1A antibody; chromatin modifying protein 1A antibody; hVps46 1 antibody; KIAA0047 antibody; PCOLN3 antibody; PRSM1 antibody; Vps46 1 antibody
This antibody was obtained by immunization of CHMP1A recombinant protein (Accession Number: BC007527). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109231(CHMP1A antibody) at dilution of 1:500
IP Result of anti-CHMP1A (IP:Catalog No:109231, 3ug; Detection:Catalog No:109231 1:500) with HEK-293 cells lysate 880ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109231(CHMP1A Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109231(CHMP1A Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:109231(CHMP1A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
- Scheffer LL, Sreetama SC, Sharma N. Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. Nature communications. 5:5646. 2014.
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