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Chk2 antibody

Cat.#: 109229

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Product Information

  • Product Name
    Chk2 antibody
  • Documents
  • Description
    Chk2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HL-60 cells. Positive IP detected in HL-60 cells. Positive IF detected in RAW 264.7 cells. Observed molecular weight by Western-blot: 65 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CDS1 antibody; CHEK2 antibody; Chk2 antibody; HuCds1 antibody; LFS2 antibody; PP1425 antibody; RAD53 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Chk2 recombinant protein (Accession Number: NM_007194). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109229(CHEK2 antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109229(CHEK2 antibody) at dilution of 1:500

    IP Result of anti-CHEK2 (IP:Catalog No:109229, 4ug; Detection:Catalog No:109229 1:500) with HL-60 cells lysate 1600ug.

    IP Result of anti-CHEK2 (IP:Catalog No:109229, 4ug; Detection:Catalog No:109229 1:500) with HL-60 cells lysate 1600ug.

    Immunofluorescent analysis of (-20oc Ethanol) fixed RAW 264.7 cells using Catalog No:109229(CHEK2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed RAW 264.7 cells using Catalog No:109229(CHEK2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.
  • References
    • Wu J, Chen W, Yin Y, Zheng Z, Zou G. Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 27(3):445-58. 2014.
    • Song X, Li L, Shi Q. Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells. Chemical research in toxicology. 28(11):2160-9. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"