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Product Name
CHIT1 Polyclonal Antibody
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Description
Polyclonal antibody to CHIT1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
CHIT1 antibody; CHI3 antibody; CHIT antibody; CHITD antibody; chitinase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CHIT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHIT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - CHIT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human normal stomach using CHIT1 antibody at dilution of 1:200 (40x lens).
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Background
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"