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Product Name
CHFR antibody
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Description
CHFR Rabbit Polyclonal antibody. Positive IHC detected in human endometrial cancer tissue, human lung cancer tissue. Positive WB detected in HepG2 cells, A549 cells, Jurkat cells, mouse brain tissue, SMMC-7721 cells. Observed molecular weight by Western-blot: 70-85 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHFR antibody; FLJ10796 antibody; FLJ33629 antibody; RING finger protein 196 antibody; RNF116 antibody; RNF196 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHFR recombinant protein (Accession Number: NM_001161346). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
WB result of Catalog No:109224(CHFR antibody).
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109224(CHFR antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:109224(CHFR antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:109224(CHFR antibody) at dilution of 1:100 (under 40x lens)
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Background
The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.
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References
- Tanaka M, Chang P, Li Y. Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 17(13):4531-40. 2011.
- Kashima L, Idogawa M, Mita H. CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. The Journal of biological chemistry. 287(16):12975-84. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"