-
Product Name
CHD4 antibody
- Documents
-
Description
CHD4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IHC detected in human cervical cancer tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 260 kDa
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ATP dependent helicase CHD4 antibody; CHD 4 antibody; CHD4 antibody; DKFZp686E06161 antibody; Mi 2b antibody; Mi2 BETA antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CHD4 recombinant protein (Accession Number: XM_006718958). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109220(CHD4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109220(CHD4 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of A549 cells, using CHD4 antibody Catalog No:109220 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182].
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"