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Product Name
CHCHD6 antibody
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Description
CHCHD6 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IHC detected in human stomach tissue. Observed molecular weight by Western-blot: 26-29 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CHCHD6 antibody; CHCM1 antibody; MIC25 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHCHD6 recombinant protein (Accession Number: NM_032343). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109215(CHCHD6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human stomach using Catalog No:109215(CHCHD6 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:109215(CHCHD6 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CHCHD6 (IP:Catalog No:109215, 4ug; Detection:Catalog No:109215 1:500) with HeLa cells lysate 1200ug.
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Background
CHCHD6 represents coiled-coil-helix-coiled-coil-helix domain containing 6
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References
- Li H, Ruan Y, Zhang K. Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation. 23(3):380-92. 2016.
- Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015.
- Guarani V, McNeill EM, Paulo JA. QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. eLife. 4:. 2015.
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