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Product Name
CHCHD3 antibody
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Description
CHCHD3 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human heart tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, mouse liver tissue. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CHCHD3 antibody; FLJ20420 antibody; MIC19 antibody; MINOS3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHCHD3 recombinant protein (Accession Number: NM_017812). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109213(CHCHD3 Antibody) at dilution of 1:1000
IP Result of anti-CHCHD3 (IP:Catalog No:109213, 4ug; Detection:Catalog No:109213 1:1000) with HeLa cells lysate 2600ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109213(CHCHD3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109213(CHCHD3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109213(CHCHD3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CHCHD3 represents coiled-coil-helix-coiled-coil-helix domain containing 3
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References
- Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015.
- Plecitá-Hlavatá L, Engstová H, Alán L. Hypoxic HepG2 cell adaptation decreases ATP synthase dimers and ATP production in inflated cristae by mitofilin down-regulation concomitant to MICOS clustering. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"