-
Product Name
CHAMP1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CHAMP1
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse
-
Alternative names
CHAMP1 antibody; C13orf8 antibody; CAMP antibody; CHAMP antibody; MRD40 antibody; ZNF828 antibody; chromosome alignment-maintaining phosphoprotein 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 563-812 of human CHAMP1 (NP_115812.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CHAMP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHAMP1 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - CHAMP1 Polyclonal Antibody
Immunofluorescence analysis of Hela cells using CHAMP1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Background
Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"