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Product Name
CHAF1B Polyclonal Antibody
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Description
Polyclonal antibody to CHAF1B
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHAF1B antibody; CAF-1 antibody; CAF-IP60 antibody; CAF1 antibody; CAF1A antibody; CAF1P60 antibody; MPHOSPH7 antibody; MPP7 antibody; chromatin assembly factor 1 subunit B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100 -
Validations
Western blot - CHAF1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHAF1B antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHAF1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody at dilution of 1:100 (40x lens).
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Background
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"