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Product Name
CES1 Polyclonal Antibody
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Description
Polyclonal antibody to CES1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
CES1 antibody; ACAT antibody; CE-1 antibody; CEH antibody; CES2 antibody; HMSE antibody; HMSE1 antibody; PCE-1 antibody; REH antibody; SES1 antibody; TGH antibody; hCE-1 antibody; carboxylesterase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 287-566 of human CES1 (NP_001257.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CES1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CES1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - CES1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using CES1 antibody at dilution of 1:100 (40x lens).
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Background
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"