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Product Name
Centrin 1 antibody
- Documents
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Description
Centrin 1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in HL-60 cells, human heart tissue. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Caltractin isoform 2 antibody; CEN1 antibody; Centrin 1 antibody; centrin antibody; EF hand protein antibody; CETN antibody; CETN1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Centrin 1 recombinant protein (Accession Number: NM_004066). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109259(CETN1 antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using CETN1 antibody Catalog No:109259 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
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References
- Wan Y, Yang Z, Guo J. Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance. Cell research. 22(7):1140-54. 2012.
- Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. The EMBO journal. 32(4):597-607. 2013.
- Zhao H, Zhu L, Zhu Y. The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. Nature cell biology. 15(12):1434-44. 2013.
- Zhang Y, Wang Y, Wei Y. The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly. Biochemical and biophysical research communications. 458(3):494-500. 2015.
- Kodani A, Yu TW, Johnson JR. Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication. eLife. 4:. 2015.
- Zhang C, Zhang W, Lu Y. NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1. Cell research. 26(2):239-53. 2016.
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