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Product Name
CENPQ Polyclonal Antibody
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Description
Polyclonal antibody to CENPQ
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CENPQ antibody; C6orf139 antibody; CENP-Q antibody; centromere protein Q antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 131-268 of human CENPQ (NP_060602.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CENPQ Polyclonal Antibody
Western blot analysis of extracts of Jurkat cells, using CENPQ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CENPQ Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CENPQ antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"