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Product Name
CENPK Polyclonal Antibody
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Description
Polyclonal antibody to CENPK
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CENPK antibody; AF5alpha antibody; CENP-K antibody; FKSG14 antibody; P33 antibody; Solt antibody; centromere protein K antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human CENPK (NP_071428.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CENPK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CENPK Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - CENPK Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CENPK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"