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Product Name
CDKN3 Polyclonal Antibody
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Description
Polyclonal antibody to CDKN3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
CDKN3 antibody; CDI1 antibody; CIP2 antibody; KAP antibody; KAP1 antibody; cyclin-dependent kinase inhibitor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CDKN3 (NP_001124323.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - CDKN3 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using CDKN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - CDKN3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using CDKN3 antibody . Blue: DAPI for nuclear staining.
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Background
May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"