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Product Name
CDK8 antibody
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Description
CDK8 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HEK-293 cells, HeLa cells. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human stomach cancer tissue, human testis tissue. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CDK8 antibody; Cell division protein kinase 8 antibody; cyclin dependent kinase 8 antibody; K35 antibody; Mediator complex subunit CDK8 antibody; Protein kinase K35 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK8 recombinant protein (Accession Number: NM_001318368). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109167(CDK8 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:109167(CDK8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:109167(CDK8 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:109167(CDK8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CDK8 represents cyclin-dependent kinase 8
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"