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Product Name
CDK6 antibody
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Description
CDK6 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, C2C12 cells, C6 cells. Positive IP detected in C6 cells. Positive IHC detected in human endometrial cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 37-40 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDK6 antibody; Cell division protein kinase 6 antibody; cyclin dependent kinase 6 antibody; PLSTIRE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK6 recombinant protein (Accession Number: BC027989). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109165(CDK6 antibody) at dilution of 1:500
C2C12 cells (shRNA samples) were subjected to SDS PAGE followed by western blot with Catalog No:109165 (CDK6 antibody) at dilution of 1:500. (Data provided by Angran Biotech (www.miRNAlab.com)).
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:109165(CDK6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:109165(CDK6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CDK6 antibody Catalog No:109165 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CDK6 (IP:Catalog No:109165, 3ug; Detection:Catalog No:109165 1:300) with C6 cells lysate 1600ug.
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Background
CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The range of the molecular weight is 34-50 KDa.
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References
- Cao J, Shen Y, Zhu L. miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Nature cell biology. 14(7):697-706. 2012.
- Dhar SS, Alam H, Li N. Transcriptional repression of histone deacetylase 3 by the histone demethylase KDM2A is coupled to tumorigenicity of lung cancer cells. The Journal of biological chemistry. 289(11):7483-96. 2014.
- Song Y, Gong K, Yan H. Sj7170, a unique dual-function peptide with a specific α-chymotrypsin inhibitory activity and a potent tumor-activating effect from scorpion venom. The Journal of biological chemistry. 289(17):11667-80. 2014.
- Ning Z, Wang A, Liang J. USP22 promotes the G1/S phase transition by upregulating FoxM1 expression via β-catenin nuclear localization and is associated with poor prognosis in stage II pancreatic ductal adenocarcinoma. International journal of oncology. 45(4):1594-608. 2014.
- Chen J, Liu G, Weng X. Tougu Xiaotong formula induces chondrogenic differentiation in association with transforming growth factor-β1 and promotes proliferation in bone marrow stromal cells. International journal of molecular medicine. 35(3):747-54. 2015.
- Bar J, Gorn-Hondermann I, Moretto P. miR Profiling Identifies Cyclin-Dependent Kinase 6 Downregulation as a Potential Mechanism of Acquired Cisplatin Resistance in Non-Small-Cell Lung Carcinoma. Clinical lung cancer. 16(6):e121-9. 2015.
- He Y, Tang D, Cai C, Chai R, Li H. LSD1 is Required for Hair Cell Regeneration in Zebrafish. Molecular neurobiology. 2015.
- Guo X, Deng K, Wang H. GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6. Oncology research and treatment. 38(7-8):362-6. 2015.
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