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Product Name
CDK5RAP3 antibody
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Description
CDK5RAP3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C53 antibody; CDK5RAP3 antibody; HSF 27 antibody; IC53 antibody; LZAP antibody; MST016 antibody; OK/SW cl.114 antibody; PP1553 antibody; Protein HSF 27 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK5RAP3 recombinant protein (Accession Number: NM_176096). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109164(CDK5RAP3 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109164(CDK5RAP3 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CDK5RAP3 antibody Catalog No:109164 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug CDK5RAP3 antibody (Catalog No:109164, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CDK5RAP3 represents CDK5 regulatory subunit associated protein 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"