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Product Name
CDK5 antibody
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Description
CDK5 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, mouse pancreas tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDK5 antibody; Cell division protein kinase 5 antibody; cyclin dependent kinase 5 antibody; PSSALRE antibody; TPKII catalytic subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK5 recombinant protein (Accession Number: NM_004935). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109162(CDK5 antibody) at dilution of 1:500
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:109162(CDK5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.
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References
- Liu W, Zhang Y, Hao J. Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. Journal of cellular biochemistry. 113(10):3186-96. 2012.
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