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Product Name
CDK16 Polyclonal Antibody
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Description
Polyclonal antibody to CDK16
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CDK16 antibody; PCTAIRE antibody; PCTAIRE1 antibody; PCTGAIRE antibody; PCTK1 antibody; cyclin-dependent kinase 16 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 277-496 of human CDK16 (NP_006192.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CDK16 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CDK16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - CDK16 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CDK16 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"