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Product Name
CDK11B Polyclonal Antibody
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Description
Polyclonal antibody to CDK11B
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CDK11B antibody; CDC2L1 antibody; CDK11 antibody; CDK11-p110 antibody; CDK11-p46 antibody; CDK11-p58 antibody; CLK-1 antibody; PITSLREA antibody; PK58 antibody; p58 antibody; p58CDC2L1 antibody; p58CLK-1 antibody; cyclin-dependent kinase 11B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human CDK11B (NP_277021.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CDK11B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CDK11B Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CDK11B Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CDK11B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Plays multiple roles in cell cycle progression, cytokinesis and apoptosis. Involved in pre-mRNA splicing in a kinase activity-dependent manner. Isoform 7 may act as a negative regulator of normal cell cycle progression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"