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Product Name
CDH15 Polyclonal Antibody
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Description
Polyclonal antibody to CDH15
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Tested applications
WB
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Species reactivity
Rat
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Alternative names
CDH15 antibody; CDH14 antibody; CDH3 antibody; CDHM antibody; MCAD antibody; MRD3 antibody; cadherin-15 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CDH15 (NP_004924.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CDH15 Polyclonal Antibody
Western blot analysis of extracts of rat brain, using CDH15 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"