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Product Name
CDC45L antibody
- Documents
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Description
CDC45L Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue. Positive IP detected in K-562 cells. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 60-66kd
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Tested applications
ELISA, WB, IF, IHC, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDC45 antibody; CDC45L antibody; CDC45L2 antibody; PORC PI 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDC45L recombinant protein (Accession Number: NM_003504). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109109(CDC45L antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using CDC45L antibody Catalog No:109109 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CDC45L (IP:Catalog No:109109, 3ug; Detection:Catalog No:109109 1:1000) with K-562 cells lysate 3400ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109109(CDC45L Antibody) at dilution of 1:50
1X10^6 HeLa cells were stained with 0.2ug CDC45L antibody (Catalog No:109109, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"