-
Product Name
CDC42EP3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CDC42EP3
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
CDC42EP3 antibody; BORG2 antibody; CEP3 antibody; UB1 antibody; cdc42 effector protein 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human CDC42EP3 (NP_006440.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - CDC42EP3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CDC42EP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"