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Product Name
CDC34 antibody
- Documents
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Description
CDC34 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in human pancreas tissue, human brain tissue. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDC34 antibody; E2 CDC34 antibody; UBC3 antibody; UBCH3 antibody; UBE2R1 antibody; Ubiquitin protein ligase R1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDC34 recombinant protein (Accession Number: NM_004359). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:109102(CDC34 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109102(CDC34 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CDC34 (IP:Catalog No:109102, 4ug; Detection:Catalog No:109102 1:500) with mouse testis tissue lysate 4000ug.
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Background
CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676).
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References
- Fiesel FC, Moussaud-Lamodière EL, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. Journal of cell science. 127(Pt 16):3488-504. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"