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CDC25C antibody

Cat.#: 109098

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Special Price 239.0 USD

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Product Information

  • Product Name
    CDC25C antibody
  • Documents
  • Description
    CDC25C Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HepG2 cells, Raji cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 53 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CDC25 antibody; CDC25C antibody; M phase inducer phosphatase 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CDC25C recombinant protein (Accession Number: XM_047417952). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109098(CDC25C antibody) at dilution of 1:800

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109098(CDC25C antibody) at dilution of 1:800

    IP Result of anti-CDC25C (IP:Catalog No:109098, 4ug; Detection:Catalog No:109098 1:1000) with K-562 cells lysate 3600ug.

    IP Result of anti-CDC25C (IP:Catalog No:109098, 4ug; Detection:Catalog No:109098 1:1000) with K-562 cells lysate 3600ug.

  • Background
    The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513).
  • References
    • Zhan W, Han T, Zhang C. TRIM59 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer Cells by Upregulating Cell Cycle Related Proteins. PloS one. 10(11):e0142596. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"