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Product Name
CDC25A antibody
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Description
CDC25A Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human gliomas tissue. Positive WB detected in HepG2 cells, mouse liver tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CDC25A antibody; CDC25A2 antibody; M phase inducer phosphatase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001789). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109096(CDC25A antibody) at dilution of 1:1500
IP Result of anti-CDC25A (IP:Catalog No:109096, 4ug; Detection:Catalog No:109096 1:300) with HeLa cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:109096(CDC25A Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:109096(CDC25A Antibody) at dilution of 1:50 (under 40x lens)
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Background
CDC25A blongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.
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