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Product Name
CD99 antibody
- Documents
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Description
CD99 Rabbit Polyclonal antibody. Positive WB detected in U-937 cells. Positive IHC detected in human pancreas tissue, human testis tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 28 kDa, 32 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
12E7 antibody; CD99 antibody; CD99 antigen antibody; CD99 molecule antibody; E2 antigen antibody; MIC2 antibody; MIC2X antibody; MIC2Y antibody; Protein MIC2 antibody; T cell surface glycoprotein E2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD99 recombinant protein (Accession Number: NM_002414). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:109088(CD99 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:109088(CD99 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 Jurkat cells were stained with 0.5ug CD99 antibody (Catalog No:109088, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:109088(CD99 Antibody) at dilution of 1:1000
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Background
CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"