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Product Name
CD79B Polyclonal Antibody
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Description
Polyclonal antibody to CD79B
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
CD79B antibody; AGM6 antibody; B29 antibody; IGB antibody; CD79b molecule antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-159 of human CD79B (NP_000617.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CD79B Polyclonal Antibody
Western blot analysis of extracts of Raji cells, using CD79B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunofluorescence - CD79B Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using CD79B antibody . Blue: DAPI for nuclear staining.
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Background
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"