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Product Name
CD79A antibody
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Description
CD79A Rabbit Polyclonal antibody. Positive WB detected in Raji cells. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD79A antibody; Ig alpha antibody; IGA antibody; MB 1 antibody; MB 1 membrane glycoprotein antibody; MB1 antibody; Surface IgM assOCiated protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD79A recombinant protein (Accession Number: NM_001783). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109138(CD79A antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109138(CD79A Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109138(CD79A Antibody) at dilution of 1:200 (under 40x lens)
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Background
CD79A, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. The calculated molecular weight of CD79A is 25 kDa, but we detected a 42 kDa in Raji cell lysate.
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