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Product Name
CD71 antibody
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Description
CD71 Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Raji cells. Positive IHC detected in human placenta tissue. Positive IF detected in HeLa cells. Positive FC detected in Raji cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, IHC, WB, IF, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CD71 antibody; p90 antibody; sTfR antibody; TFR antibody; TFR1 antibody; TFRC antibody; Transferrin receptor protein 1 antibody; TRFR antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of CD71 recombinant protein (Accession Number: NM_001128148). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107143(CD71 Antibody) at dilution of 1:2000
1X10^6 Raji cells were stained with 0.2ug CD71 antibody (Catalog No:107143, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:100.
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:107143(CD71 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:107143(CD71 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:107143(CD71 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
IP Result of anti-CD71 (IP:Catalog No:107143, 5ug; Detection:Catalog No:107143 1:1000) with HeLa cells lysate 2400ug.
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Background
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"