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Product Name
CD69 antibody
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Description
CD69 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Activation inducer molecule antibody; AIM antibody; BL AC/P26 antibody; CD69 antibody; CD69 molecule antibody; CLEC2C antibody; EA1 antibody; Early activation antigen CD69 antibody; GP32/28 antibody; MLR 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD69 recombinant protein (Accession Number: NM_001781). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:50-1:500
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109135(CD69 antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109135(CD69 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109135(CD69 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.
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