-
Product Name
CD63 antibody
- Documents
-
Description
CD63 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IF detected in A431 cells. Positive IHC detected in human malignant melanoma tissue, human lymphoma tissue, human tonsillitis tissue.
-
Tested applications
ELISA, IHC, WB, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
CD63 antibody; CD63 antigen antibody; CD63 molecule antibody; Granulophysin antibody; LAMP 3 antibody; ME491 antibody; MLA1 antibody; OMA81H antibody; Tetraspanin 30 antibody; Tspan 30 antibody; TSPAN30 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CD63 recombinant protein (Accession Number: NM_001257390). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using Catalog No:109133(CD63 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using Catalog No:109133(CD63 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed A431 cells using Catalog No:109133(CD63 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109133(CD63 Antibody) at dilution of 1:1000
-
Background
CD63, known as LAMP-3, is a lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"