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Product Name
CD59 antibody
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Description
CD59 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human placenta tissue. Positive IF detected in BxPC-3 cells. Positive FC detected in Jurkat cells. Positive WB detected in BxPC-3 cells, human brain tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
16.3A5 antibody; 1F5 antibody; 1F5 antigen antibody; CD59 antibody; CD59 glycoprotein antibody; EJ16 antibody; EJ30 antibody; EL32 antibody; FLJ38134 antibody; FLJ92039 antibody; G344 antibody; HRF 20 antibody; HRF20 antibody; MAC inhibitory protein antibody; MAC IP antibody; MACIF antibody; MEM43 antibody; MEM43 antigen antibody; MIC11 antibody; MIN1 antibody; MIN2 antibody; MIN3 antibody; MIRL antibody; MSK21 antibody; p18 20 antibody; Protectin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD59 recombinant protein (Accession Number: NM_001127223). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109131(CD59 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109131(CD59 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of BxPC-3 cells using Catalog No:109131(CD59 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Jurkat cells were stained with 0.5ug CD59 antibody (Catalog No:109131, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25kd. It acts as a determinant of proximal–distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
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References
- Wang Y, Wang R, Jiang S. Gecko CD59 is implicated in proximodistal identity during tail regeneration. PloS one. 6(3):e17878. 2011.
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