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Product Name
CD5 antibody
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Description
CD5 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive IP detected in mouse liver tissue. Positive WB detected in Jurkat cells, mouse liver tissue. Observed molecular weight by Western-blot: 55 kDa,67 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CD5 antibody; CD5 molecule antibody; LEU1 antibody; LymphOCyte antigen T1/Leu 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD5 recombinant protein (Accession Number: NM_014207). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109128(CD5 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109128(CD5 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109128(CD5 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
IP Result of anti-CD5 (IP:Catalog No:109128, 4ug; Detection:Catalog No:109128 1:500) with mouse liver tissue lysate 6800ug.
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Background
CD5 is a cell surface glycoprotein expressed on a majority of thymocytes, mature T cells and a subset of B cells called B-1 cells. CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"