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Product Name
CD43 antibody
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Description
CD43 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive WB detected in Jurkat cells, K-562 cells. Observed molecular weight by Western-blot: 115 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
CD43 antibody; Galactoglycoprotein antibody; GALGP antibody; GPL115 antibody; LeukOCyte sialoglycoprotein antibody; Leukosialin antibody; LSN antibody; sialophorin antibody; SPN antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of CD43 recombinant protein (Accession Number: NM_001030288). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107135(SPN Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using (SPN Antibody) at dilution of 1:100 (under 10x lens). . Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using (SPN Antibody) at dilution of 1:100 (under 40x lens). . Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
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Background
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
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