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Product Name
CD42b antibody
- Documents
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Description
CD42b Rabbit Polyclonal antibody. Positive WB detected in A431 cells, Jurkat cells, SH-SY5Y cells. Positive IP detected in A431 cells. Positive IHC detected in human spleen tissue. Observed molecular weight by Western-blot: 80-85 kDa,145 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
BSS antibody; CD42b antibody; CD42b alpha antibody; GP1B antibody; GP1BA antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD42b recombinant protein (Accession Number: BC027955). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109122(GP1BA antibody) at dilution of 1:500
IP Result of anti-GP1BA (IP:Catalog No:109122, 3ug; Detection:Catalog No:109122 1:500) with A431 cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109122(GP1BA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109122(GP1BA antibody) at dilution of 1:50 (under 40x lens)
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Background
GP1BA, also named as CD42b , is a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB. It is 145kd in platelet. It is Glycocalicin and phopho and showed 80-85kd in Jurkat.
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References
- Blaschitz A, Siwetz M, Schlenke P, Gauster M. Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta. Placenta. 36(11):1333-6. 2015.
- Siwetz M, Blaschitz A, El-Heliebi A. TNF-α alters the inflammatory secretion profile of human first trimester placenta. Laboratory investigation; a journal of technical methods and pathology. 2016.
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