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Product Name
CD34 antibody
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Description
CD34 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue, human liver cancer tissue, human placenta tissue. Positive WB detected in human placenta tissue. Observed molecular weight by Western-blot: 105-120 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD34 antibody; CD34 molecule antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of CD34 recombinant protein (Accession Number: NM_001025109). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107132(CD34 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107132(CD34 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107132(CD34 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD34 is a surface glycophosphoprotein expressed on developmentally early lymphohematopoietic stem and progenitor cells with a molecular weight of about 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
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