CD3 gamma antibody
CD3 gamma Rabbit Polyclonal antibody. Positive FC detected in Jurkat cells. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive WB detected in mouse thymus tissue, Jurkat cells. Positive IP detected in mouse thymus tissue. Observed molecular weight by Western-blot: 20-25 kDa
ELISA, WB, FC, IHC, IP
Human,Mouse,Rat; other species not tested.
CD3 gamma antibody; CD3G antibody; CD3-gamma antibody; T cell receptor T3 gamma chain antibody; T3G antibody
This antibody was obtained by immunization of CD3 gamma recombinant protein (Accession Number: NM_000073). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
1X10^6 Jurkat cells were stained with 0.5ug CD3G antibody (Catalog No:109021, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109021(CD3G antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109021(CD3G antibody) at dilution of 1:50 (under 40x lens)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:109021(CD3G antibody) at dilution of 1:500
IP Result of anti-CD3G (IP:Catalog No:109021, 4ug; Detection:Catalog No:109021 1:600) with mouse thymus tissue lysate 2800ug.
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
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