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CD27 antibody

Cat.#: 109016

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD27 antibody
  • Documents
  • Description
    CD27 Rabbit Polyclonal antibody. Positive FC detected in Raji cells. Positive WB detected in Raji cells. Observed molecular weight by Western-blot: 50 kDa
  • Tested applications
    ELISA, WB, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD27 antibody; CD27 antigen antibody; CD27 molecule antibody; CD27L receptor antibody; S152 antibody; T cell activation antigen CD27 antibody; T14 antibody; TNFRSF7 antibody; Tp55 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD27 recombinant protein (Accession Number: NM_001242). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109016(CD27 antibody) at dilution of 1:300

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109016(CD27 antibody) at dilution of 1:300

    1X10^6 Raji cells were stained with 0.2ug CD27 antibody (Catalog No:109016, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Raji cells were stained with 0.2ug CD27 antibody (Catalog No:109016, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (Cd27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"