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Product Name
CD27 antibody
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Description
CD27 Mouse Monoclonal antibody. Positive WB detected in Ramos cells, Daudi cells. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD27 antibody; CD27 antigen antibody; CD27 molecule antibody; CD27L receptor antibody; S152 antibody; T cell activation antigen CD27 antibody; T14 antibody; TNFRSF7 antibody; Tp55 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of CD27 recombinant protein (Accession Number: NM_001242). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
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Validations
Ramos cells were subjected to SDS PAGE followed by western blot with Catalog No:107127(CD27 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107127(CD27 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107127(CD27 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (Cd27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.
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