CD247 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive FC detected in Jurkat cells. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, human spleen tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 16 kDa
ELISA, WB, FC, IP, IHC
Human,Mouse,Rat; other species not tested.
CD247 antibody; CD247 molecule antibody; CD3 ZETA antibody; CD3H antibody; CD3Q antibody; CD3Z antibody; T cell receptor T3 zeta chain antibody; T3Z antibody; TCRZ antibody
This antibody was obtained by immunization of CD247 recombinant protein (Accession Number: NM_198053). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109015(CD247 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109015(CD247 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109015(CD247 Antibody) at dilution of 1:200 (under 40x lens).
1X10^6 Jurkat cells were stained with 0.5ug CD247 antibody (Catalog No:109015, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-CD247 (IP:Catalog No:109015, 3ug; Detection:Catalog No:109015 1:700) with Jurkat cells lysate 1200ug.
CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
- Kaczmarek J, Homsi Y, van Üüm J. Liver sinusoidal endothelial cell-mediated CD8 T cell priming depends on co-inhibitory signal integration over time. PloS one. 9(6):e99574. 2014.
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