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CD2 antibody

Cat.#: 109065

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD2 antibody
  • Documents
  • Description
    CD2 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive FC detected in Jurkat cells. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 45-50 kDa
  • Tested applications
    ELISA, WB, FC, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD2 antibody; CD2 molecule antibody; ErythrOCyte receptor antibody; FLJ46032 antibody; LFA 2 antibody; LFA 3 receptor antibody; Rosette receptor antibody; SRBC antibody; T cell surface antigen CD2 antibody; T11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD2 recombinant protein (Accession Number: NM_001767). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109065(CD2 antibody) at dilution of 1:1000

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109065(CD2 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109065(CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109065(CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109065(CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109065(CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    1X10^6 Jurkat cells were stained with 0.2ug CD2 antibody (Catalog No:109065, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug CD2 antibody (Catalog No:109065, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"