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Product Name
CD164 antibody
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Description
CD164 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive FC detected in K-562 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 82 kDa
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human; other species not tested.
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Alternative names
CD164 antibody; CD164 molecule antibody; sialomucin antibody; endolyn antibody; MUC 24 antibody; Sialomucin core protein 24 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD164 recombinant protein (Accession Number: NM_006016). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: N/AIHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109060(CD164 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109060(CD164 antibody) at dilution of 1:50 (under 10x lens)
1X10^6 K-562 cells were stained with 0.2ug CD164 antibody (Catalog No:109060, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"