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CD14 antibody

Cat.#: 109053

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD14 antibody
  • Documents
  • Description
    CD14 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human appendix tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive FC detected in RAW 264.7 cells. Positive WB detected in HL-60 cells, human spleen tissue, RAW 264.7 cells, RAW264.7 cells, U-937 cells. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CD14 antibody; CD14 molecule antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD14 recombinant protein (Accession Number: NM_001174104). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109053(CD14 Antibody) at dilution of 1:600

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109053(CD14 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109053(CD14 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109053(CD14 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109053(CD14 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:109053(CD14 Antibody) at dilution of 1:200 (under 40x lens).

    1X10^6 RAW 264.7 cells were stained with 0.2ug CD14 antibody (Catalog No:109053, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 RAW 264.7 cells were stained with 0.2ug CD14 antibody (Catalog No:109053, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.
  • References
    • Mishra N, Nugent WH, Mahavadi S, Walsh SW. Mechanisms of enhanced vascular reactivity in preeclampsia. Hypertension. 58(5):867-73. 2011.
    • Wang C, Yuan J, Wu HX. Total glucosides of paeony inhibit the inflammatory responses of mice with allergic contact dermatitis by restoring the balanced secretion of pro-/anti-inflammatory cytokines. International immunopharmacology. 24(2):325-34. 2015.
    • Liu T, Men Q, Wu G. Tetrandrine induces autophagy and differentiation by activating ROS and Notch1 signaling in leukemia cells. Oncotarget. 6(10):7992-8006. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"