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Product Name
CD13 antibody
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Description
CD13 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, human liver tissue. Positive IHC detected in human kidney tissue, human colon tissue. Positive FC detected in HEK-293 cells. Observed molecular weight by Western-blot: 150 kDa, 135 kDa, 110 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alanyl aminopeptidase antibody; Aminopeptidase M antibody; Aminopeptidase N antibody; ANPEP antibody; AP M antibody; AP N antibody; APN antibody; CD13 antibody; gp150 antibody; hAPN antibody; LAP1 antibody; Microsomal aminopeptidase antibody; p150 antibody; PEPN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD13 recombinant protein (Accession Number: XM_011521473). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109046(ANPEP antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:109046(ANPEP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:109046(ANPEP Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HEK-293 cells were stained with 0.2ug ANPEP antibody (Catalog No:109046, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CD13 is also named as APN, ANPEP(aminopeptidase N) , PEPN and belongs to the peptidase M1 family. It is a 150-kD cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells.CD13 is an important regulator of angiogenesis where its expression on activated blood vessels is induced by angiogenic signals.
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References
- RamachandraRao SP, Matthias MA, Kokoy-Mondragon C. Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats. PLoS neglected tropical diseases. 9(3):e0003640. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"