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Product Name
CD127/IL7R antibody
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Description
CD127/IL7R Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, K-562 cells. Positive IP detected in K-562 cells. Positive FC detected in K-562 cells. Observed molecular weight by Western-blot: 56-58 kDa
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Tested applications
ELISA, WB, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CD127 antibody; CDW127 antibody; IL 7 receptor subunit alpha antibody; IL 7R alpha antibody; IL 7R subunit alpha antibody; IL 7RA antibody; IL7R antibody; IL7RA antibody; ILRA antibody; interleukin 7 receptor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD127/IL7R recombinant protein (Accession Number: NM_002185). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:109045(IL7R antibody) at dilution of 1:1500
IP Result of anti-IL7R (IP:Catalog No:109045, 4ug; Detection:Catalog No:109045 1:1000) with K-562 cells lysate 1200ug.
1X10^6 K-562 cells were stained with 0.2ug IL7R antibody (Catalog No:109045, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"