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Product Name
CD11B Polyclonal Antibody
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Description
Polyclonal antibody to CD11B
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ITGAM antibody; CD11B antibody; CR3A antibody; MAC-1 antibody; MAC1A antibody; MO1A antibody; SLEB6 antibody; integrin alpha-M antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11B (NP_001139280.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - CD11B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CD11B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - CD11B Polyclonal Antibody
Immunofluorescence analysis of rat bone marrow using CD11B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CD11B Polyclonal Antibody
Immunofluorescence analysis of RAW264.7 cells using CD11B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CD11B Polyclonal Antibody
Immunofluorescence analysis of THP-1 cells using CD11B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"