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Product Name
CCT8 Polyclonal Antibody
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Description
Polyclonal antibody to CCT8
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCT8 antibody; C21orf112 antibody; Cctq antibody; D21S246 antibody; PRED71 antibody; T-complex protein 1 subunit theta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 248-497 of human CCT8 (NP_001269837.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CCT8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CCT8 antibody at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Western blot - CCT8 Polyclonal Antibody
Western blot analysis of extracts of various cells, using CCT8 antibody at 1:7000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - CCT8 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CCT8 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
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